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RT-LAMP could be used for SARS-CoV-2 testing and overcomes the challenges of adapting an assay to a point-of-care instrument. Further, the reduced instrumentation cost and complexity, along with the simple workflow, highlight the potential for implementation of RT-LAMP for SARS-CoV-2 testing in resource-limited settings.
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Here we provide detailed technical data and evaluate the performance of three novel rRT-PCR assays targeting the ORF1ab, N, and E genes for detection of COVID-19 infection. The application of rRT-PCR assays among four types of specimens (alveolar lavage, sputum, throat swabs, and stool) from patients with COVID-19 indicated that the mean viral.
Refonte du site Relais Colis pour le B2C et le B2B
Jung-Chung Lin b Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei,. In particular, using real-time RT-PCR with the lab-developed PCR assay and the BD Max system, we detected 55 samples as positive and 150 samples as negative for SARS-CoV-2..
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The RNAzol RT reagent was used to provide pure RNA from human cells. We develop a protocol using RNAzol RT reagent to extract pure RNA from plants tissues and demonstrate that this RNA extraction method works not only at room temperature but also at elevated temperatures and provides the simplest and most effective single-step method to extract pure and undegraded RNA directly from tropical.
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The use of RT-LAMP (reverse transcriptase—loop mediated isothermal amplification) has been considered as a promising point-of-care method to diagnose COVID-19. In this manuscript we show that.
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The developed protocol was applied to one-step dPCR, which allowed the detection of 1,130.2 RNA copies/µL using 10 −2 μg/nL of viral particles with a 30 min thermolysis-RT reaction at 70°C ( Table 5 ). The protocol developed by optimizing RT-qPCR analysis was verified and applied to dPCR analysis.
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Plant materials. To develop single and multiplex RT-LAMP assays, we collected plant material from various Musa genotypes growing at the Waimanalo Research Station, College of Tropical Agriculture and Human Resources (CTAHR), University of Hawaii at Manoa, located in Honolulu County, HI, USA. The material included twelve samples from plants with typical symptoms of banana bunchy top disease.
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The real-time RT-PCR master mixture in a 25 μl volume was optimized, and the mixture consisted of 12.5 μl of 2 × one-step RT-PCR buffer, 0.5 μl of Ex Taq HS, 0.5 μl of RT Enzyme Mix II, 0.5 μl of each primer and probe, 5 μl of RNA/DNA template, and finally RNase-free water was added for a total volume of 25 μl in each reaction tube.
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The global COVID-19 pandemic has highlighted the need for rapid, accurate and accessible nucleic acid tests to enable timely identification of infected individuals. We optimized a sample-to-answer nucleic acid test for SARS-CoV-2 that provides results in <1 hour using inexpensive and readily available reagents. The test workflow includes a simple lysis and viral inactivation protocol followed.
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Analysis of the results, in comparison to RT-qPCR, found that the ZIKV RT-LAMP assay provided sensitivity of 100%, specificity of 93.75%, and an overall accuracy of 95.00%.
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In 2019 a newly identified coronavirus, designated as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread rapidly from the epicenter in Wuhan (China) to more than 150 countries around the world, causing the Coronavirus disease 2019 (COVID-19) pandemic. In this study, we describe an extraction-less method based on reverse transcriptase loop-mediated isothermal amplification.
